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The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.

Identifieur interne : 003306 ( Main/Exploration ); précédent : 003305; suivant : 003307

The use of RAPD and semi-random markers to verify somatic hybrids between diploid lines of Solanum tuberosum L.

Auteurs : Jarosław Przetakiewicz [Pologne] ; Anna Nadolska-Orczyk ; Wacław Orczyk

Source :

RBID : pubmed:12378226

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English descriptors

Abstract

Markers specific to diploid lines of cultivated potato were identified using the polymerase chain reaction (PCR), primed with arbitrary 10-mers generating RAPDs, or with semi-random primers targeting intron-exon semi-conservative sequences of plant genes. One hundred and fifty RAPD primers and twelve semi-random primers were tested. Selected primers were subsequently used for verification of putative somatic hybrids.

PubMed: 12378226


Affiliations:


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<name sortKey="Nadolska Orczyk, Anna" sort="Nadolska Orczyk, Anna" uniqKey="Nadolska Orczyk A" first="Anna" last="Nadolska-Orczyk">Anna Nadolska-Orczyk</name>
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<term>Solanum tuberosum (génétique)</term>
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